| 1. | Aerodynamic gene gun for dna immunization
用于基因疫苗接种的气动式基因枪 |
| 2. | Here we have used dye injection and gene gun labeling to identify and follow their differentiation in both normal and dark reared rabbits
本篇研究主要是用微量注射和基因枪标定技术，观察在全暗饲养下和正常光照饲养下兔子双极细胞的分化情形有何不同。 |
| 3. | ( 1 ) the intermediate vector pjar harbors acti promoter , regulating rip gene , and bar gene as selectable marker . this vector was used in gene gun transformation
( 1 )中间载体pjar含act1启动子调控的rip基因，选择标记基因为bar基因，可用于基因枪转化。 |
| 4. | There are many routes of dna vaccine immunization , including intramuscular injection , intradermal injection , subcutaneous injection , gene gun immunization , mucosa immunization , and so on
核酸疫苗的接种有多种途径,包括肌内、皮内、皮下注射途径、基因枪免疫、黏膜免疫途径等。 |
| 5. | The tpsl gene was introguced into immature embryo cells of 12 - 16 days wheat cultivars wen . 6 via gene gun delivery technique . resistant calli were selected on medium containing hygromin ( 40 - 60mg / l )
用基因枪将海藻糖- 6 -磷酸合成酶基因( tps1 )导入小麦栽培品种温6的12 - 16日龄的幼胚细胞中。 |
| 6. | Strawberry calli were transformed by particle bombardment ( pds - 1000 / he gene gun ) with plasmid pby520 containing late embryogenesis abundant protein gene , lea3 , from barley . lea3 gene was regulated by act i and pin ii , bar gene as a selected gene
)的胚胎发生晚期丰富蛋白lea3基因的pby520质粒dna导入草莓愈伤组织细胞( act为启动子， pin为终止子， bar基因为选择基因) 。 |
| 7. | ( 2 ) establishment of maize transformation system material r18 transformation system were established and transgentic plants of bar gene were obtained uing gene gun . statistical analysis showed that r18 performent well with yd culture medium in the process of conservation , screening and regeneration
( 2 )玉米遗传转化体系的建立及抗除草剂转基因植株的获得于2000年选用r18为受体材料，用基因枪法导入bar基因(抗除草剂草丁膦) ，建立起了遗传转化体系。 |
| 8. | The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii . two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium . the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome . at the same time ; compared agrabactenum - mediated method with gene gun method , the transformation frequency of the former was 16 . 7 % , while the latter was 50 % , so gene gun transformation method was suitable for long ya liiliwn
用携带有几丁质酶基因和- 1 、 3葡聚糖酶基因的工程菌，通过农杆菌介导法和基因枪转化法转化龙牙百合，经pcr和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较，发现农杆菌介导法的转化率为16 . 7 ，基因枪法的转化率为50 ，因此可能基因枪转化法更适于龙牙百合的遗传转化。 |
| 9. | The fusion ( f ) protein and hemagglutinin - neuraminidase ( hn ) are the major virulence factors of the virus to which the host responds well with protective antibody production . recombinant plasmids containing the f or hn gene can induce specific immune responses against ndv . however , dna vaccination by injection or gene gun routes is rather inconvenient and costly in field applications in scaled animal farms
融合蛋白( f )和血凝素-神经氨酸酶蛋白( hn )是ndv的主要宿主保护性抗原，以f基因和hn基因为免疫原研制的dna疫苗能诱导一定程度的免疫应答，但注射免疫或基因枪免疫途径因实际操作和成本等问题难以推广应用，而直接口服重组质粒dna免疫效果不理想。 |
| 10. | Based on cdna of tps1 gene , pcambia1303 plasmid , we have succeeded to construct plant reombinant vector phfsm001transforming the construct plant expressing vector to immature of wheat embryo by gene gun , the wheat plant possessing the tpsl gene is attained by tissue culture and hygromylin selected
本文以tps1基因的cdna ，质粒p ~ ( cambia1303 )为研究对象，运用基因克隆技术，成功构建了植物表达载体phfjm001用基因枪转化小麦幼胚，经组织培养和潮霉素筛选得到了含有海藻糖合成酶基因的转基因小麦植株。 |
